Survival of tubercle bacilli in heat-fixed sputum smears.
نویسندگان
چکیده
منابع مشابه
Survival of tubercle bacilli in heat-fixed sputum smears.
Tubercle bacilli, which survived heat fixation, were detected with a slide culture technique which allowed the entire smear to be examined. Both conventional flame fixation and the use of a controlled hot-plate failed to render tuberculous sputum smears safe for further handling. Smears which were stained with the phenol-auramine method failed to yield growth on culture. If delay between prepa...
متن کاملSurvival of tubercle bacilli in heat-fixed and stained sputum smears.
We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in...
متن کاملExamination of smears for tubercle bacilli by fluorescence microscopy.
I n underdeveloped countries, laboratory faci l i ties for the bacterio logical d iagnosis of tubercu losis are, at present , very l imited . Cultural methods are un l i kely to be used on a large scale for many years to come. It is , therefore, important to investigate the most economical method of examin ing smears for tubercle baci l l i . Fluorescence microscopy was introduced by H agemann...
متن کاملAutomated identification of tubercle bacilli in sputum. A preliminary investigation.
OBJECTIVE To use an automated method to detect tubercle bacilli in sputum specimens. STUDY DESIGN Using fluorescence microscopy, tubercle bacilli were identified on auramine-stained sputum specimens. Images were then captured with a digital camera and enhanced through imaging processing techniques. The bacilli were recognized using neural network classifiers. RESULTS This preliminary invest...
متن کاملImproved polyacrylamide-based artificial sputum with formalin-fixed tubercle bacilli for training of tuberculosis microscopists.
Sputum smear microscopy is an easy, inexpensive, and rapid method for detecting tubercle bacilli when there are more than 10,000 bacilli/ml in the original sputum. Furthermore, because the microscopic method provides not only quantitative, but also qualitative information, such as the shape of bacilli, it has remained significant. We have previously developed and reported panel test slides made...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1981
ISSN: 0021-9746
DOI: 10.1136/jcp.34.7.719